Directional PCR Cloning of Multiple Repeat Sequences
نویسندگان
چکیده
منابع مشابه
Directional PCR cloning of multiple repeat sequences.
The tandem ligation of DNA sequences within plasmid vectors is hindered by the inability to force the orientation of subsequently ligated DNA fragments and the inherent instability of repeated sequences when grown in bacterial hosts. Improvements have been made in the latter by the introduction of several E. coli strains by commercial suppliers (STBL2; Life Technologies, Gaithersburg, MD, USA o...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1996
ISSN: 0736-6205,1940-9818
DOI: 10.2144/96212bm05